Composite

Part:BBa_K1911007:Design

Designed by: Jackson Harris   Group: iGEM16_LambertGA   (2016-10-21)


pLamdaR-LacI-GFP-pLac-ClpXP-CI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1257
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1221
    Illegal BglII site found at 3074
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2556
    Illegal AgeI site found at 3294
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1900


Design Notes

We had to make sure there weren't any illegal restriction enzyme sites. Double terminators and double stop codons were added appropriately so that translation and transcription didn't continue to express random DNA sequences.

Source

The construct was created by combining parts found on the iGEM registry. It is a composite part created through the 3A assembly.

References